Polyclonal Anti Histidine

Polyclonal Anti Histidine

Cat #: 652501

Size: 100 ul

Source: Biolegend

Application: WB – Quality tested ICC, ICFC – Validated

Made by: DNAbiotech.co



This antibody was raised against linear 8XHis tag. It reacts with His tag protein, either C, N, or internal His6 and up repeats.

It can be used for Western blotting to detect linear His repeat (His6 or more). It can also be used for ICFC and IF staining of His-Tagged protein.

Mouse IgG1, κ Isotype
Histidine Tag Other Names
All species (6x His tag epitope) Reactivity
Monoclonal Antibody Type
Mouse Host Species
KLH-conjugated His-tag peptide Immunogen
This antibody is provided in phosphate-buffered solution, pH 7.2, 0.09% NaN3 Formulation
The antibody was purified by affinity chromatography. Preparation
0.5 mg/ml Concentration
Upon receipt, store undiluted between 2°C and 8°C Storage & Handling

Recommended Usage:

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 – 0.5 µg per ml (Dilution 1:1000 – 1:5000). For immunocytochemistry, a concentration range of 0.1 – 0.5 μg/ml (Dilution 1:1000 – 1:5000) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Function: Recombinant poly-histidine tag detection