ELISA stop solution
Cat #: DB9690-25ml
size: 25 ml
Packeging: Dark glass bottle
Made by DNAbiotech Co. IR Iran
Data Sheet: click here
The reaction can be stopped by addition of stop reagent, and the color turns from blue to yellow with 2-3 fold increased absorbance values at 450 nm.
ELISA Stop Solution contains 4N HCL. Suitable for use in micro well assays such as ELISA. ELISA Stop Solution is suitable to stop the TMB enzyme substrate reaction in ELISA.
Each lot of Stop Solution for TMB (3,3’5,5′-tetramethylbenzidine) Substrate has been tested by ELISA. The TMB stop solution will stop TMB and HRP (horseradish peroxidase) reaction by adding the same volume as the TMB substrate added to each well.
Addition of 50 ul stop solution changes the color from blue to yellow. Read absorbance at 450 nm within 60 minutes. If the reader can read at 570 nm, the absorbance at 570 nm can be subtracted from the absorbance at 450 nm.
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