ELISA stop solution

ELISA stop solution

Cat #: DB9690-25ml

size: 25 ml

Packeging: Dark glass bottle

Made by DNAbiotech Co. IR Iran

Data Sheet: click here


Stop Solution for TMB Substrate is specifically formulated to terminate the HRP/TMB reaction and stabilizing the color development for ELISA applications. TMB-based substrates turn yellow upon addition of TMB Stop Solution.

The reaction can be stopped by addition of stop reagent, and the color turns from blue to yellow with 2-3 fold increased absorbance values at 450 nm.


• Stop Solution (4N HCL) for ELISA assay

ELISA Stop Solution contains 4N HCL. Suitable for use in micro well assays such as ELISA. ELISA Stop Solution is suitable to stop the TMB enzyme substrate reaction in ELISA.

 Application : ELISA
Storage : 1 year at room temperature

Application Notes:

Each lot of Stop Solution for TMB (3,3’5,5′-tetramethylbenzidine) Substrate has been tested by ELISA. The TMB stop solution will stop TMB and HRP (horseradish peroxidase) reaction by adding the same volume as the TMB substrate added to each well.

Addition of 50 ul stop solution changes the color from blue to yellow. Read absorbance at 450 nm within 60 minutes. If the reader can read at 570 nm, the absorbance at 570 nm can be subtracted from the absorbance at 450 nm.

Related products:

Goat anti Rabbit, HRP conjugated (DB9572)

Goat anti Mouse, HRP conjugated (DB9571)

TMB substrate kit (DB9510)

Goat anti Human, HRP conjugated (DB9573)